Identifying Micro-Organisms

AIM: to practice using some sterile techniques, to substantiate a range of micro-organisms found in bloodline macroscopically and to examine a range of micro- organisms found in pabulum and water supply macroscopically APPARATUS:         Incubator          sterilized nutritive agar plates 1.         3 water ensamples 2.         1 air sample 3.         3 food samples          etna burner         Sterile pipettes          protest loops         Sticky tape         Marking tape         Water samples(various)         yogurt         Inoculating needle/ probe          sick from fruit, vegetables or wampumpeag         Methylated strong drink RISK ASSESMENT: 1.         Glassw atomic number 18 - tin cause cuts if handled incorrectly 2.         Buns en burner - can cause burn down if not on caoutchouc flare 3.         Methylated booze - toxic if ingested, highly combustible. Skin irritant 4.         Although the micro-organisms utilize in the activities in this manual are considered safe, standard microbiological appendage requires that they are regarded as potentially harmful SAFETY PROCEDURES: 1.         Handle with care, do not surrender alone glassware near the shore of the bench 2.         take care, do not leave flame unattended keep flammable liquid i.e. Methylated strong drink away 3.         Keep Methylated spirits away from flames. stick out gloves when handling it 4.         Wear gloves, lab coat and safety glasses. every last(predicate) used equipment should be placed in disinfectant. Dispose of cultures afterward use, in an autoclave. Wash hands forrader leaving lab. never open agar plate after setting up METHOD: 1.         Sterilise the workbench ! area by soak up alcohol on to it 2.         collect 8 sterile nutrient agar plates 3.         A sample of mellisonant milk was obtained 4.         leave one plate aside, certain and taped as the curb 5.          use a sterile inoculating loop, a undersized sample of the fresh milk was streaked onto the plates. This plate was indeed sealed with sticky tape and labeled as fresh milk 6.          travel 3 and 4 were then perennial with tap water, distilled water, seek tank water, fresh milk, old milk, If you inadequacy to get a full essay, order it on our website: OrderEssay.net

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