Introduction:
In this investigation, it result be determined whether the changing substratum niggardness (through dilution of 3% atomic number 1 peroxide) has an effect on the stray reception with catalase derive (of beef liver).
The substrate that will be apply is hydrogen peroxide (H2O2). An enzyme which accelerates the breakdown of hydrogen peroxide into water and atomic number 8 gas is known as a catalase.
2H2O2--------catalase--------------> 2H2O + O2
A cognitive content which decreases the activation energy of a chemical reaction and augment the rate of reaction is a catalyst. So a catalase is an organic fertiliser catalyst. In this experiment, hydrogen peroxide will be used as the substrate for the catalase.
Enzyme activity is proportionally to substrate slow-wittedness at low substrate concentration. At higher substrate concentration there are more collisions involving the substrate and energetic site. Since the active sites are all taken up in high substrate concentration for the enzyme, when the substrate concentration is increase there is no effect.
The graph below shows the change in the rate of reaction with higher substrate concentration, where the rate of reaction reaches a peak then is leveled off:
Source: hypertext transfer protocol://www.rsc.org/education/teachers/learnnet/cfb/enzymes.htm
In this experiment, the substrate concentration will be manipulated by diluting the 3% hydrogen peroxide. Furthermore, the initial reaction rate with the catalase extract (beef liver) will be determined by the type O output in intervals of 20 seconds for a period of 3 minutes (180 seconds).
Research Question:
What is the affect of the change in substrate concentration (dilution of 3% hydrogen peroxide) on the rate of rate with catalase extract (beef liver)?
Hypothesis:
As the substrate concentration (of...
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